Part:BBa_J73999:Design

plasmid vector for Rhodobacter sphaeroides

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NotI site found at 2468
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 4636
  Illegal XhoI site found at 28
  Illegal XhoI site found at 3463
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 4620
  Illegal NgoMIV site found at 4644
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 513
  

Design Notes

To make this vector more flexible and easier to control, we have deleted the lacZ promoter and lac reporter and made it a standard BioBrick vector.

Source

From pMG170/pMG171